Cucurbitacin B

Research use only, not for human use.

Cucurbitacin B has profound in vitro and in vivo antiproliferative effects against human pancreatic Y cells. It inhibited AKT signaling activation through up-regulation of PTEN.

Cucurbitacin B is a plant-derived triterpene that has the classic four-ring structure of mammalian steroids. Cucurbitacin B suppresses metastasis mediated by reactive oxygen species (ROS) via focal adhesion kinase (FAK) in breast cancer MDA-MB-231 cells. Cucurbitacin B induces inhibitory effects via CIP2A/PP2A/Akt pathway in glioblastoma multiforme. Cucurbitacin B acts a potential insect growth regulator by antagonizing 20-hydroxyecdysone activity.

Cell Viability Assay Cell Line:CCA cell lines Concentration:0.1, 1 0.5, 1,5, 10, 20, 40 μM Incubation Time:24 and 48 h Result:Decreased cell viability in a dose-dependent and time-dependent manner with the IC50 values of 13:44 μM for 24 h and 1.55 for 48 h.Cell Cycle Analysis Cell Line:CCA cell lines Concentration:0.1, 1, 10 μM Incubation Time:24 h Result:Arrested cell cycle progression at the G2/M phase.Western Blot Analysis Cell Line:CCA cell lines Concentration:0.1, 1 0.5, 1,5, 10, 20, 40 μM Incubation Time:12 and 24 h Result:Decreased the expression of Cyclin A, Cyclin D1, Cdc25A, but increased the level of p21.

Animal Model:Carrageenan-induced prostatic inflammation in rats Dosage:5mg/kg/day for 10day Administration:Oral administration Result:Decreased the level of TNF-α, IL-1b, COX-2 and iNOS in prostatic tissues.Animal Model:STZ-ICV rat prototype of AD-like dementia Dosage:20, 50mg/kg/day for 28days Administration:Intraperitoneal injection (i.p.)Result:Decreased the TNF-α, IL-1β, MPO, iNOS, acetylcholinesterase, and glutamate levels, but increased gamma-aminobutyric acid. Increase in viable neuron density in the cortex and hippocampus of rats.

Cucurbitacin B | Cuc B | NSC 49451

GPCR/G Protein

Hedgehog (Hh)

HIF-1| PTEN| STAT3

Cancer

——

6199-67-3

558.7

C32H46O8

>98% (HPLC)

DMSO : ≥ 100 mg/mL 178.99 mM; H2O : < 0.1 mg/mL

C(=O)(C)OC(C)(/C=C/C(=O)[C@](C)(O)[C@H]1[C@@H](C[C@]2([C@@H]3CC=C4C(C(=O)[C@H](C[C@H]4[C@@]3(C(=O)C[C@]12C)C)O)(C)C)C)O)C

(R,E)-6-((2S,8S,9R,10R,13R,14S,16R,17R)-2,16-dihydroxy-4,4,9,13,14-pentamethyl-3,11-dioxo-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl)-6-hydroxy-2-methyl-5-oxohept-3-en-2-yl acetate

(-20℃)

With Ice Pack

≥ 2 years